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Genetic engineering and protein biochemistry - CIGEx

​​UMR1274

Published on 30 August 2019

​​​​​​The Cellule d’Ingenierie Genetique et d’Expression (CIGEx) is open to national academic scientific community.
​The CIGEx offers services ranging from molecular biology steps (cloning of gene of interest, genetic engineering) to protein biochemistry (interaction test, recombinant protein purification).

The CIGEx has been implemented with the goals ​​​​of :

  • providing efficient tools and technologies with well established know-how,
  • providing LIMS and quality control at all steps,
  • providing scientific and technological environment for users,
  • providing training for students and researchers​.

Didier BUSSO
Principal investigator
Phone : + 33 (0)1 46 54 91 42
didier.busso@cea.fr

Secretary of UMR 1274

Aurélie GOURET
Phone : +33 (0)1 46 54 98 66

Arielle LELIARD
Phone : +33 (0)1 46 54 99 49
Services

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  • Molecular cloning of gene of interest using different technologies (GatewayTM, SLIC, Gibson-CloningTM, Restriction-ligation).
  • ​Genetic engineering of plasmids.
  • Recombinant protein production in different expression systems (E. coli and insect cells infected by baculovirus).
  • Screen for expression, co-expression and solubility conditions.
  • Interaction tests by “pull-down” (IMAC, GST) or by immuno-precipitations.
  • Expression, co-expression and purification of recombinant proteins.


Recent production