Bioscience and Biotechnology Institute of Aix-Marseille
Energies | Renewable energies | Bioenergy | Biofuel | Biohydrogen | Microalgae | Photosynthesis
To select the strains of interest, the laboratory has developed two screening methods. The first one discriminates on the lipid composition, the second on the photosynthetic activity of the organism.
Step 1 :
Step 2 :
Step 3 :
Step 4 :
Step 5 :
By GC-FID or GC-M
Publication of the screening method :
Cagnon C, Mirabella B, Nguyen HM, Beyly-Adriano A, Bouvet S, Cuiné S, Beisson F, Peltier G, Li-Beisson Y. A genetic screen to isolate oil mutants in the green microalga Chlamydomonas reinhardtii . Biotechnol. Biofuels 6, 178
A system allowing to follow the
fluorescence in time, on plates of culture, was finalized with the cooperation of Bernard Genty and Illia Reiters (LEMP-CEA Cadarache).This device based on the acquisition by a fluorescent camera of the signal of fluorescence and an excitement by a crown of diodes allowed to screen a bank of more than 3000 mutants of
Chlamydomonas to isolate
mutants (Ghysel and al .).
CEA is a French government-funded technological research organisation in four main areas: low-carbon energies, defense and security, information technologies and health technologies. A prominent player in the European Research Area, it is involved in setting up collaborative projects with many partners around the world.