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LGBP

Technologies

Published on 22 May 2015

​Expertise and Equipment

The laboratory benefits from a unique combination of broad knowledge, equipment and methods that cover biophysics, biochemistry, molecular and cellular biology. In addition to the standard equipment for molecular biology and biochemistry, our laboratory equipment includes ultracentrifuges, an HPLC, an FPLC, a laser spectrometer, an FTIR spectrometer, a Joliot spectrophotometer, spectrophotometers, s spectrofluorometer, a Fusion FX7 chemiluminescence/bioluminescence/fluorescence video imaging system, a PAM and video-imaging fluorometer, a Bioruptor Next Gen Sonicator, and an HRM-compatible qPCR machine. Additionally we have easy access to state-of-the-art microscopy (http://www.ibdm.univ-mrs.fr/technology/imaging-microscopy/) and genomic (http://tagc.univ-mrs.fr/tagc/index.php/tgml-facility) facilities on the Luminy campus.​

 

in vitro growth room
Crédit : M. Reissolet/ CNRS
 
 
 
 
 
Fluorcam.
Crédit : S. Caffarri,/ Aix-Marseille Université

 

HPLC
Crédit : S. Caffarri, Aix-Marseille Université

Plant growth facilities

The LGBP currently has 7 controlled environmental growth rooms totalling about 54 square meters of growth space. Five of these rooms are specifically designed for growing plants in soil and are equipped with computerized environmental controls that control temperature, lighting, humidity and irrigation. One large room is specifically designed for in vitro culture and cell suspensions. One cold room (16°C) is available for Physcomitrella patens gametophore production. Facilities also include 2 Percival AR‐41L3 plant growth chambers for specific experiments as well as incubators and orbital shakers for cultures in liquid media .

 

Photosynthesis analysis platform

LGBP has a photosynthesis analysis platform, coupled to the Plant Growth Facilities, for the comprehensive analysis of photosynthetic parameters and pigments.  :

  • PAM-type fluorometer (model dual PAM 100/F, WALZ). A PAM fluorometer is the device of reference for the analysis of photosynthetic parameters using chlorophyll fluorescence in different photosynthetic organisms. It can be applied to both intact tissues (plants) or preparations in solution (chloroplasts, algae, bacteria)..
  • Fluorescence video imaging system (model Fluorcam FC 800-O, Photon System Instruments). The Fluorcam is equipped with a CCD camera detector and allows the simultaneous measurement of chlorophyll fluorescence in multiple samples. This system is indispensable for high throughput genetic screens of mutagenized populations for the identification of mutants carrying particular photosynthetic phenotypes. The system also allows the measurement of the GFP fluorescence in photosynthetic / non-photosynthetic organisms.

    Spectrofluorometer Cary Eclipse (Varian)

    The Cary Eclipse fluorescence spectrometer uses a Xenon flash lamp for high sensitivity and fast kinetics. It measures the emission of light from samples in four modes (fluorescence, phosphorescence, chemi/bio-luminescence, and time resolved phosphorescence). Measurements at low temperature (liquid nitrogen) are also possible.​