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Clonage et criblage des activités de bioconversions


Publié le 5 janvier 2016
Synthesis of Chiral Amines via a Bi-Enzymatic Cascade Using an Ene-Reductase and Amine Dehydrogenase
Jongkind EPJ, Fossey-Jouenne A, Mayol O, Zaparucha A, Vergne-Vaxelaire C and Paul CE
Change in Cofactor Specificity of Oxidoreductases by Adaptive Evolution of an Escherichia coli NADPH-Auxotrophic Strain
Bouzon M, Doring V, Dubois I, Berger A, Stoffel GMM, Ramirez LC, Meyer SN, Foure M, Roche D, Perret A, Erb TJ, Bar-Even A and Lindner SN
Biocatalysed synthesis of chiral amines: continuous colorimetric assays for mining amine-transaminases
Gourbeyre L, Heuson E, Charmantray F, Helaine V, Debard A, Petit JL, de Berardinis V and Gefflaut T
Complete Genome Sequences of Two Pseudomonas Species Isolated from Marine Environments of the Pacific Ocean
Wang SZ, Cruaud C, Aury JM, Vallenet D, Poulain J, Vacherie B, Zaparucha A and Vergne-Vaxelaire C
Metagenomic Mining for Amine Dehydrogenase Discovery
Caparco AA, Pelletier E, Petit JL, Jouenne A, Bommarius BR, de Berardinis V, Zaparucha A, Champion JA, Bommarius AS and Vergne-Vaxelaire C
Pyruvate Aldolases Catalyze Cross-Aldol Reactions between Ketones: Highly Selective Access to Multi-Functionalized Tertiary Alcohols
Laurent V, Gourbeyre L, Uzel A, Helaine V, Nauton L, Traikia M, de Berardinis V, Salanoubat M, Gefflaut T, Lemaire M and Guerard-Helaine C
ATP Regeneration System in Chemoenzymatic Amide Bond Formation with Thermophilic CoA Ligase
Lelievre CM, Balandras M, Petit JL, Vergne-Vaxelaire C and Zaparucha A
Continuous Culture Adaptation of Methylobacterium extorquens AM1 and TK 0001 to Very High Methanol Concentrations
Belkhelfa S, Roche D, Dubois I, Berger A, Delmas VA, Cattolico L, Perret A, Labadie K, Perdereau AC, Darii E, Pateau E, de Berardinis V, Salanoubat M, Bouzon M and Doring V
2-Deoxyribose-5-phosphate aldolase, a remarkably tolerant aldolase towards nucleophile substrates
Chambre D, Guerard-Helaine C, Darii E, Mariage A, Petit JL, Salanoubat M, de Berardinis V, Lemaire M and Helaine V
Simplified in Vitro and in Vivo Bioaccess to Prenylated Compounds
Couillaud J, Rico J, Rubini A, Hamrouni T, Courvoisier-Dezord E, Petit JL, Mariage A, Darii E, Duquesne K, de Berardinis V and Iacazio G
XszenFHal, a novel tryptophan 5-halogenase from Xenorhabdus szentirmaii
Domergue J, Erdmann D, Fossey-Jouenne A, Petit JL, Debard A, de Berardinis V, Vergne-Vaxelaire C and Zaparucha A
Enantioselective Synthesis of D- and L-alpha-Amino Acids by Enzymatic Transamination Using Glutamine as Smart Amine Donor
Heuson E, Charmantray F, Petit JL, de Berardinis V and Gefflaut T
Discovery of new levansucrase enzymes with interesting properties and improved catalytic activity to produce levan and fructooligosaccharides
Hill A, Chen L, Mariage A, Petit JL, de Berardinis V and Karboune S
Achiral Hydroxypyruvaldehyde Phosphate as a Platform for Multi-Aldolases Cascade Synthesis of Diuloses and for a Quadruple Acetaldehyde Addition Catalyzed by 2-Deoxyribose-5-Phosphate Aldolases
Laurent V, Helaine V, Vergne-Vaxelaire C, Nauton L, Traikia M, Petit JL, Salanoubat M, de Berardinis V, Lemaire M and Guerard-Helaine C
Exploration of Aldol Reactions Catalyzed by Stereoselective Pyruvate Aldolases with 2-Oxobutyric Acid as Nucleophile
Laurent V, Uzel A, Helaine V, Nauton L, Traikia M, Gefflaut T, Salanoubat M, de Berardinis V, Lemaire M and Guerard-Helaine C
A family of native amine dehydrogenases for the asymmetric reductive amination of ketones
Mayol O, Bastard K, Beloti L, Frese A, Turkenburg JP, Petit JL, Mariage A, Debard A, Pellouin V, Perret A, de Berardinis V, Zaparucha A, Grogan G and Vergne-Vaxelaire C
Tuning of the enzyme ratio in a neutral redox convergent cascade: A key approach for an efficient one-pot/two-step biocatalytic whole-cell system
Menil S, Petit JL, Courvoisier-Dezord E, Debard A, Pellouin V, Reignier T, Sergent M, Deyris V, Duquesne K, de Berardinis V and Alphand V
Exploring natural biodiversity to expand access to microbial terpene synthesis
Rico J, Duquesne K, Petit JL, Mariage A, Darii E, Peruch F, de Berardinis V and Iacazio G
Synthesis of Branched-Chain Sugars with a DHAP-Dependent Aldolase: Ketones are Electrophile Substrates of Rhamnulose-1-phosphate Aldolases
Laurent V, Darii E, Aujon A, Debacker M, Petit JL, Helaine V, Liptaj T, Breza M, Mariage A, Nauton L, Traikia M, Salanoubat M, Lemaire M, Guerard-Helaine C and de Berardinis V
Elucidation of the trigonelline degradation pathway reveals previously undescribed enzymes and metabolites
Perchat N, Saaidi PL, Darii E, Pelle C, Petit JL, Besnard-Gonnet M, de Berardinis V, Dupont M, Gimbernat A, Salanoubat M, Fischer C and Perret A
Single-cell genomics of multiple uncultured stramenopiles reveals underestimated functional diversity across oceans
Seeleuthner Y, Mondy S, Lombard V, Carradec Q, Pelletier E, Wessner M, Leconte J, Mangot JF, Poulain J, Labadie K, Logares R, Sunagawa S, de Berardinis V, Salanoubat M, Dimier C, Kandels-Lewis S, Picheral M, Searson S, Pesant S, Poulton N, Stepanauskas R, Bork P, Bowler C, Hingamp P, Sullivan MB, Iudicone D, Massana R, Aury JM, Henrissat B, Karsenti E, Jaillon O, Sieracki M, de Vargas C, Wincker P and Tara Oceans C
Characterization of a thermotolerant ROK-type mannofructokinase from Streptococcus mitis: application to the synthesis of phosphorylated sugars
Vergne-Vaxelaire C, Mariage A, Petit JL, Fossey-Jouenne A, Guerard-Helaine C, Darii E, Debard A, Nepert S, Pellouin V, Lemaire M, Zaparucha A, Salanoubat M and de Berardinis V
Parallel evolution of non-homologous isofunctional enzymes in methionine biosynthesis
Bastard K, Perret A, Mariage A, Bessonnet T, Pinet-Turpault A, Petit JL, Darii E, Bazire P, Vergne-Vaxelaire C, Brewee C, Debard A, Pellouin V, Besnard-Gonnet M, Artiguenave F, Medigue C, Vallenet D, Danchin A, Zaparucha A, Weissenbach J, Salanoubat M and De Berardinis V
Biocatalytic Approaches towards the Synthesis of Chiral Amino Alcohols from Lysine: Cascade Reactions Combining alpha-Keto Acid Oxygenase Hydroxylation with Pyridoxal Phosphate- Dependent Decarboxylation
Baud D, Peruch O, Saaidi PL, Fossey A, Mariage A, Petit JL, Salanoubat M, Vergne-Vaxelaire C, de Berardinis V and Zaparucha A
Expanding the reaction space of aldolases using hydroxypyruvate as a nucleophilic substrate
de Berardinis V, Guerard-Helaine C, Darii E, Bastard K, Helaine V, Mariage A, Petit JL, Poupard N, Sanchez-Moreno I, Stam M, Gefflaut T, Salanoubat M and Lemaire M
Stereoselective synthesis of gamma-hydroxy-alpha-amino acids through aldolase-transaminase recycling cascades
Guerard-Helaine C, Heuson E, Ndiaye M, Gourbeyre L, Lemaire M, Helaine V, Charmantray F, Petit JL, Salanoubat M, de Berardinis V and Gefflaut T
One-pot, two-step cascade synthesis of naturally rare L-erythro (3S,4S) ketoses by coupling a thermostable transaminase and transketolase
Lorilliere M, De Sousa M, Bruna F, Heuson E, Gefflaut T, de Berardinis V, Saravanan T, Yi D, Fessner WD, Charmantray F and Hecquet L
Continuous colorimetric screening assays for the detection of specific l- or d-alpha-amino acid transaminases in enzyme libraries
Heuson E, Petit JL, Debard A, Job A, Charmantray F, de Berardinis V and Gefflaut T
Asymmetric reductive amination by a wild-type amine dehydrogenase from the thermophilic bacteria Petrotoga mobilis
Mayol O, David S, Darii E, Debard A, Mariage A, Pellouin V, Petit JL, Salanoubat M, de Berardinis V, Zaparucha A and Vergne-Vaxelaire C
Osmotic stress response in Acinetobacter baylyi: identification of a glycine-betaine biosynthesis pathway and regulation of osmoadaptive choline uptake and glycine-betaine synthesis through a choline-responsive BetI repressor
Scholz A, Stahl J, de Berardinis V, Muller V and Averhoff B
Genetic Dissection of the Type VI Secretion System in Acinetobacter and Identification of a Novel Peptidoglycan Hydrolase, TagX, Required for Its Biogenesis
Weber BS, Hennon SW, Wright MS, Scott NE, de Berardinis V, Foster LJ, Ayala JA, Adams MD and Feldman MF
Genome Mining for Innovative Biocatalysts: New Dihydroxyacetone Aldolases for the Chemist's Toolbox
Guerard-Helaine C, de Berardinis V, Besnard-Gonnet M, Darii E, Debacker M, Debard A, Fernandes C, Helaine V, Mariage A, Pellouin V, Perret A, Petit JL, Sancelme M, Lemaire M and Salanoubat M
Straightforward Synthesis of Terminally Phosphorylated L-Sugars via Multienzymatic Cascade Reactions
Helaine V, Mahdi R, Babu GVS, de Berardinis V, Wohlgemuth R, Lemaire M and Guerard-Helaine C
Design of Artificial Metabolisms in Layered Nanomaterials for the Enzymatic Synthesis of Phosphorylated Sugars
Mahdi R, Guerard-Helaine C, Prevot V, de Berardinis V, Forano C and Lemaire M
Revealing the hidden functional diversity of an enzyme family
Bastard K, Smith AAT, Vergne-Vaxelaire C, Perret A, Zaparucha A, De Melo-Minardi R, Mariage A, Boutard M, Debard A, Lechaplais C, Pelle C, Pellouin V, Perchat N, Petit JL, Kreimeyer A, Medigue C, Weissenbach J, Artiguenave F, De Berardinis V, Vallenet D and Salanoubat M
Synthesis of Mono- and Dihydroxylated Amino Acids with New alpha-Ketoglutarate-Dependent Dioxygenases: Biocatalytic Oxidation of C-H Bonds
Baud D, Saaidi PL, Monfleur A, Harari M, Cuccaro J, Fossey A, Besnard M, Debard A, Mariage A, Pellouin V, Petit JL, Salanoubat M, Weissenbach J, de Berardinis V and Zaparucha A
Large alpha-aminonitrilase activity screening of nitrilase superfamily members: Access to conversion and enantiospecificity by LC-MS
Bordier F, Stam M, Darii E, Tricot S, Fossey A, Rohault J, Debard A, Mariage A, Pellouin V, Petit JL, Perret A, Vallenet D, Salanoubat M, Weissenbach J, Vergne-Vaxelaire C, de Berardinis V and Zaparucha A
Broadening the scope of Baeyer-Villiger monooxygenase activities toward alpha,beta-unsaturated ketones: a promising route to chiral enol-lactones and ene-lactones
Reignier T, de Berardinis V, Petit JL, Mariage A, Hamze K, Duquesne K and Alphand V
Novel metabolic features in Acinetobacter baylyi ADP1 revealed by a multiomics approach
Stuani L, Lechaplais C, Salminen AV, Segurens B, Durot M, Castelli V, Pinet A, Labadie K, Cruveiller S, Weissenbach J, de Berardinis V, Salanoubat M and Perret A
Thermostable Transketolase from Geobacillus stearothermophilus: Characterization and Catalytic Properties
Abdoul-Zabar J, Sorel I, Helaine V, Charmantray F, Devamani T, Yi D, de Berardinis V, Louis D, Marliere P, Fessner WD and Hecquet L
Structure and Biosynthesis of Fimsbactins A-F, Siderophores from Acinetobacter baumannii and Acinetobacter baylyi
Proschak A, Lubuta P, Grun P, Lohr F, Wilharm G, De Berardinis V and Bode HB
Evolution study of the Baeyer-Villiger monooxygenases enzyme family: Functional importance of the highly conserved residues
Rebehmed J, Alphand V, de Berardinis V and de Brevern AG
Nitrilase Activity Screening on Structurally Diverse Substrates: Providing Biocatalytic Tools for Organic Synthesis
Vergne-Vaxelaire C, Bordier F, Fossey A, Besnard-Gonnet M, Debard A, Mariage A, Pellouin V, Perret A, Petit JL, Stam M, Salanoubat M, Weissenbach J, De Berardinis V and Zaparucha A
Microbial urate catabolism: characterization of HpyO, a non-homologous isofunctional isoform of the flavoprotein urate hydroxylase HpxO
Michiel M, Perchat N, Perret A, Tricot S, Papeil A, Besnard M, de Berardinis V, Salanoubat M and Fischer C
FSAB: A new fructose-6-phosphate aldolase from Escherichia coli. Cloning, over-expression and comparative kinetic characterization with FSAA
Sanchez-Moreno I, Nauton L, Thery V, Pinet A, Petit JL, de Berardinis V, Samland AK, Guerard-Helaine C and Lemaire M
Complete Genome Sequence of Streptomyces cattleya NRRL 8057, a Producer of Antibiotics and Fluorometabolites
Barbe V, Bouzon M, Mangenot S, Badet B, Poulain J, Segurens B, Vallenet D, Marliere P and Weissenbach J
Journal of Bacteriology 193 (18), 5055-5056, 2011
Salt adaptation in Acinetobacter baylyi: identification and characterization of a secondary glycine betaine transporter
Sand M, de Berardinis V, Mingote A, Santos H, Gottig S, Muller V and Averhoff B
Archives of Microbiology 193 (10), 723-730, 2011
Biocatalysis, bioconversion and white technology: tools from the living world for chemistry
Zaparucha A, de Berardinis V and Weissenbach J
Actualite Chimique (353-54), 43-50, 2011
A saturated SSR/DArT linkage map of Musa acuminata addressing genome rearrangements among bananas
Hippolyte I, Bakry F, Seguin M, Gardes L, Rivallan R, Risterucci AM, Jenny C, Perrier X, Carreel F, Argout X, Piffanelli P, Khan IA, Miller RNG, Pappas GJ, Mbeguie-A-Mbeguie D, Matsumoto T, De Bernardinis V, Huttner E, Kilian A, Baurens FC, D'Hont A, Cote F, Courtois B and Glaszmann JC
Bmc Plant Biology 10 (), 65, 2010
Digital expression profiling of novel diatom transcripts provides insight into their biological functions
Maheswari U, Jabbari K, Petit JL, Porcel BM, Allen AE, Cadoret JP, De Martino A, Heijde M, Kaas R, La Roche J, Lopez PJ, Martin-Jezequel V, Meichenin A, Mock T, Parker MS, Vardi A, Armbrust EV, Weissenbach J, Katinka M and Bowler C
Genome Biology 11 (8), R85, 2010
Acinetobacter baylyi ADP1 as a model for metabolic system biology
de Berardinis V, Durot M, Weissenbach J and Salanoubat M
Current Opinion in Microbiology 12 (5), 568-576, 2009
Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress
Dittami SM, Scornet D, Petit JL, Segurens B, Da Silva C, Corre E, Dondrup M, Glatting KH, Konig R, Sterck L, Rouze P, Van de Peer Y, Cock JM, Boyen C and Tonon T
Genome Biology 10 (6), 51, 2009