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Laboratory | Chemistry | High-throughput screening


LMC

14C-labeling laboratory

Published on 12 April 2017
Welcome to the website of 14C-labeling group! We are an international and enthusiastic group working at the frontiers of organic chemistry. We are located in Saclay (20 min away from Paris) at CEA, the French Alternative Energies and Atomic Energy Commission.

Feel free to browse through our website and read about who we are, our research interests and our publications.

Our laboratory is member of the ISOTOPICS European consortium.

 
French Version

Group Leader
Frédéric Taran
33 1 69 08 26 85
frederic.taran@cea.fr

Human Resources
Frédéric TARAN, Group Leader
Davide AUDISIO, Researcher
Sophie DEZARD, Researcher
Dominique GEORGIN, Researcher
Olivier LOREAU, Researcher
Sandra GABILLET, Research Technician
Nathalie CAMUS, Postdoctoral Fellow
Jijy ELIYAN, Postdoctoral Fellow
Natalie FRESNEAU, Postdoctoral Fellow
Antoine SALLUSTRAU, Postdoctoral Fellow
Sabrina BERNARD, PhD student
Elodie DECUYPERE, PhD student
Lucie PLOUGASTEL, PhD student​ 
Margaux RIOMET, PhD student​

 Group Members

    
Group picture 2016 © CEA
 

LMC Group © CEA​


Research

The focus of our research is the discovery of high throughput reactions, the development of new tools for bio-orthogonal chemistry (also known as "click") and the investigation of reliable methodologies for carbon-14 labeling of biologically active small molecules and relevant nanoparticles.

High Throughput Reaction Development



The discovery of new chemical reactions is a long-standing
goal of organic chemists. Recently, forced serendipity approaches have emerged as valuable alternative to purely rational approaches.

Over the last decade, we developed an unprecedented sandwich immunoassay screening which enables the high-throughput discovery and optimization of catalytic reactions.

This method, which allows the screening of more than 1000 catalysts or reaction conditions in a single day, has been successfully applied to new reaction discovery as well as asymmetric catalysis. 

 

Bio-orthogonal Chemistry

 

The development of chemical reactions that can be performed in living systems (i.e. cells, model organisms) has long held unique fascination in the field of chemical biology.

A bio-orthogonal reaction is characterized by the reaction of two functionalities, which will react under mild physiological conditions and are inert towards the biological environment. Quintessential example is the copper-catalyzed azide-alkyne cycloadditions (CuAAC).
We have recently applied the concept of chelation-assisted copper catalysis to the development of new azides that display unprecedented reactivity in the CuAAC, reacting almost instantaneously with alkynes under diluted conditions and in complex biological media.

Driven by our in-house sandwich immunoassays high-throughput screening, we identified two new click reactions: the Copper-catalyzed Sydnone-Alkyne Cycloaddition (CuSAC) and the Strain-Promoted Sydnone-Alkyne Cycloaddition (SPSAC).

 

Carbon-14 Labeling

Carbon-14 isotopic labeling is a longstanding interest of our research group. Carbon-14 radiolabelling is a common procedure frequently used to understand the distribution, metabolism, toxicity of a drug in vivo, in pre-clinical and early clinical studies (Phase 0/1). As part of FDA’s safety assessment process, more than 80% of all drugs have used radioactive materials in their testing program.
Our efforts in this field are focused on the development of new multi-step chemical procedures able to increase the labelling efficiency of biologically active molecule, as well as to reduce the production of radioactive waste.

We are also actively involved in the development of new methodologies for 14C-labeling of nanoparticules and nanotubes in order to study their metabolism, in vivo biodistribution and toxicity.

​Publications