Frédéric Joliot Institute for Life Sciences - On the same theme https://joliot.cea.fr/drf/joliot/en/Pages/ARCHIVES/IBITECS_anglais/News/2015/How-does-mobile-DNA-sequence-find-target.aspx Website of the Frédéric-Joliot Institute of Life Sciences which includes 4 departments: I2BC, DMTS, NeuroSpin and Service hospitalier Frédéric Joliot (SHFJ). The teams are mainly located at the CEA Paris-Saclay center and at the Orsay hospital. en-US https://www.cea.frhttps://www-cea-fr.admsite.extra.cea.fr/Style%20Library/Themes/drf/instituts/images/logo-cea-joliot.png Frédéric Joliot Institute for Life Sciences - On the same theme https://joliot.cea.fr/drf/joliot/en Rapid genome replication during early vertebrate development: role of the Rif1 protein https://www.cea.fr/drf/joliot/en/Pages/news/Science/2023/Rapid-genome-replication-role-of-Rif1-protein.aspx I2BC researchers show that the Rif1 protein restrains the replication program and the recruitment of replication initiation factors during early embryonic divisions in X laevis. A step towards elucidating the molecular mechanisms involved in diseases resulting from Rif1 mutations in humans Molecular mechanisms ; DNA SBIGEM Scientific result ; Article Thu, 21 Dec 2023 11:00:00 GMT Phytic acid: a small molecule that helps repair DNA breaks https://www.cea.fr/drf/joliot/en/Pages/news/Science/2023/phytic-acid-DNA-repair.aspx In an article published in the journal Nucleic Acids Research, scientists from the I2BC, lPBS and the Institute for Structural and Chemical Biology (University of Leicester, UK) demonstrate how phytic acid, a product of cellular metabolism, stabilises the assembly of a complex enabling the repair of DNA breaks in humans. DNA ; Structural biology Researchers SB2SM Scientific result ; Article Mon, 04 Dec 2023 11:00:00 GMT A new role for the Mediator of transcription in chromatin organization at gene promoter regions https://www.cea.fr/drf/joliot/en/Pages/news/Science/2023/New-role-Mediator-transcription-chromatin-organization.aspx A team from I2BC has shown that the Mediator of transcription from the yeast S.cerevisiae physically interacts with the chromatin remodeling complex, and that by co-localizing with this complex on DNA promoter regions, it contributes to chromatin organization favorable to transcription initiation. Health & life sciences ; DNA ; Molecular mechanisms SBIGEM Scientific result Mon, 24 Jul 2023 10:00:00 GMT A mathematical model applicable to nuclear processes at the genome scale, and at very high spatial resolution https://www.cea.fr/drf/joliot/en/Pages/news/Science/2022/Mathematical-model-repair-genome.aspx The protocols applied for in vivo mapping of nuclear processes, at the genome scale and at very high spatial resolution, do not allow for high temporal resolution. A team from the I2BC has therefore demonstrated that such an understanding could be wrong by using a mathematical model. This model can be applied to any process that can be modeled by a transition between two states. DNA ; Cellular mechanisms Researchers IBITECS Scientific result XR-seq;réparation de l’ADN;NER; Tue, 13 Dec 2022 11:00:00 GMT The Mediator of transcription at the heart of gene expression regulation and DNA repair https://www.cea.fr/drf/joliot/en/Pages/news/Science/2022/Mediator-transcription-gene-expression-regulation-DNA-repair.aspx An I2BC team has analyzed, at the genome scale of the yeast S.cerevisiae, the functional interactions between the Mediator of gene transcription and DNA repair proteins, reinforcing the idea that these two fundamental cellular processes are closely linked. Molecular mechanisms ; Cellular mechanisms ; DNA SBIGEM Scientific result Mon, 26 Sep 2022 10:00:00 GMT Accelerating mutation accumulation studies with microfluidics https://www.cea.fr/drf/joliot/en/Pages/news/Science/2021/accumulation-mutations-microfluidique.aspx Teams from CEA-Iramis and CEA-Joliot (team "Genome transcriptional regulation /I2BC) have joined forces to develop and validate an innovative microfluidic-based system that automatizes cell culture over many generations. It enables with considerable time savings large-scale experiments to be carried out, which are essential for understanding the cellular mechanisms involved in DNA mutation events. DNA ; Cellular mechanisms Researchers Iramis Scientific result Fri, 11 Jun 2021 10:00:00 GMT Phosphorylation of BAF alters its dynamics and interaction with DNA but not its contacts with the nuclear envelope https://www.cea.fr/drf/joliot/en/Pages/news/Science/2021/phosphorylation-BAF-interaction.aspx ​A study by researchers from I2BC department shows in vitro the effect of mitotic phosphorylation of the BAF protein on its structure and its interactions with DNA and lamina and unveils consequences on genome organization and on mechanisms responsible for progeroid syndromes. Molecular mechanisms ; DNA ; Structural biology Researchers SB2SM Scientific result NMR;radiocristallography; Mon, 29 Mar 2021 10:00:00 GMT A mobile laboratory to study the genetic material of extinct…or emerging species https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Mobile-laboratory-study-genetic-material-extinct-species.aspx A collaboration between researchers from I2BC@Saclay, the french Muséum National d'Histoire Naturelle and owners of prehistoric sites shows the possibility of using a mobile laboratory to quickly identify DNA of archaeological specimens. Evolution ; DNA Scientific result Wed, 08 Apr 2020 10:00:00 GMT How the transcriptional machinery recruits a DNA repair protein? https://www.cea.fr/drf/joliot/en/Pages/news/Science/2019/Transcription-and-DNA-repair.aspx Recent work by SBIGeM (I2BC@Saclay) highlights how two essential components of DNA transcription into RNA coordinate to recruit the Rad2/XPG DNA repair protein, thus linking these two fundamental cellular processes. Molecular mechanisms ; DNA Scientific result Thu, 22 Aug 2019 10:00:00 GMT DNA repair: in-depth look at the initial stages https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/DNA-repair-zoom-first-steps.aspx A research team from the SB2SM (I2BC@Saclay), in collaboration with the CNRS, SOLEIL , Universities of Paris-Sud, Aix-Marseille and Paul Sabatier - Toulouse III and Gustave Roussy, has resolved structures of two complexes involved in radiation-induced DNA damages. These results were published in Nature Structural & Molecular Biology on October, the 5th. DNA ; Structural biology ; Molecular mechanisms SB2SM Press release Mon, 08 Oct 2018 10:00:00 GMT What is the relationship between variants of the BRCA1 gene and susceptibility to cancer? https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/relationship-variants-BRCA1-cancer.aspx Research Teams from the Curie Institute, the Gustave Roussy Institute, as well as the biophysics teams from the Institute of Chemistry of Natural Substances and SB2SM (I2BC@Saclay) met to systematically identify structural defects and BRCA1 proteins encoded by variants of the BRCA1 gene, a gene predisposing to breast and ovarian cancer. Their results make it possible to propose a classification of these variants (neutral versus causal). Cancer ; Structural biology ; DNA Scientific result Fri, 28 Sep 2018 10:00:00 GMT An advance in the understanding of exchanges between DNA molecules during recombination https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/advance-exchanges-recombination.aspx Collaborative work between the SBIGeM (I2BC@Saclay) and the Marseille Cancer Research Center, published in Molecular Cell, illuminates the mechanistic basis and genetic control of homologous recombination, a mechanism for repairing DNA breaks responsible for chromosome exchange during meiosis. However, canonical models do not explain the complexity of meiotic recombination profiles and this study, which reconstructs reaction intermediates, constitutes a major advance in the understanding of this mechanism. DNA ; Genomics News Tue, 15 May 2018 10:00:00 GMT Transcription regulation by the Mediator: recent progress https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/transcription-regulation-mediator.aspx Julie Soutourina (I2BC@Saclay / SBIGeM) was invited by Nature Reviews Molecular Cell Biology to publish a review on the molecular functioning of the Mediator, a multi-protein complex, conserved from yeast to humans, and essential to the regulation of the expression of genes. Potential therapeutic approaches targeting the Mediator (cancer, fungal infections) are also adressed. Health & life sciences ; Gene and cell therapy Scientific result Wed, 17 Jan 2018 11:00:00 GMT Mediator independently orchestrates multiple steps of preinitiation complex assembly in vivo https://www.cea.fr/drf/joliot/en/Pages/ARCHIVES/IBITECS_anglais/News/2016/Mediator_transcriptional_regulation.aspx ​A genomic study of the role of the Mediator of transcriptional regulation, carried out by a SBIGeM team, shows that this multiprotein complex coordinate the multiple stages of the setting up of the transcriptional machinery in vivo.​​ DNA News Wed, 12 Apr 2017 13:23:19 GMT A new vision of the in vivo function of the transcription mediator https://www.cea.fr/drf/joliot/en/Pages/news/Science/2017/A_new_vision_of_the_in_vivo_function_of_the_transcription_mediator.aspx An in vivo, in vitro and in silico study of the Mediator of Transcriptional Regulation has shown how the function of this multiprotein complex is coordinated with another essential component of the transcription machinery, the TFIIB factor, and the architecture of the promoter. This study, led by SBIGeM, was conducted in collaboration with several other Research Teams (CNRS, CEA, INRA). DNA ; Health & life sciences Scientific result Tue, 31 Jan 2017 11:00:00 GMT Modulating cerebral cholesterol: a new avenue of research to treat Alzheimer’s disease? https://www.cea.fr/drf/joliot/en/Pages/ARCHIVES/I2BM_anglais/News/Scientific_news/Articles/2015/Modulating-cerebral-cholesterol-new-avenue-research-treat-Alzheimer.aspx Two French research teams have recently shown in a rodent model that overexpression of an enzyme capable of eliminating excess cholesterol in the brain can act in a beneficial way on the tau component of the disease to completely correct it. Alzheimer's disease ; Gene and cell therapy ; Biotechnology MIRcen Scientific result Mon, 14 Sep 2015 10:00:00 GMT Hug1 is an intrinsically disordered protein that inhibits ribonucleotide reductase activity by directly binding Rnr2 subunit. https://www.cea.fr/drf/joliot/en/Pages/ARCHIVES/IBITECS_anglais/News/2015/Hug1_and_RNR.aspx ​A study, coordinated by a SBIGeM team in collaboration with a SB2SM team, allowed to describe the role of the Hug1 protein in regulating the activity of the ribonucleotide reductase complex (RNR). The level of active RNR is controlled during cell cycle and in response to DNA damages. DNA ; Stress response ; Cell division News Mon, 04 May 2015 10:00:00 GMT How does a mobile DNA sequence find its target? https://www.cea.fr/drf/joliot/en/Pages/ARCHIVES/IBITECS_anglais/News/2015/How-does-mobile-DNA-sequence-find-target.aspx Certain mobile DNA sequences shape genomes and are maintained over the course of generations. How is this explained? The interaction between two proteins, which is essential for their integration into a specific area of the yeast genome, has been identified. This finding has interesting implications for gene therapy. DNA ; Gene and cell therapy Saclay Scientific result Fri, 01 May 2015 10:00:00 GMT Parkinson’s disease: a major breakthrough thanks to gene therapy https://www.cea.fr/drf/joliot/en/Pages/ARCHIVES/I2BM_anglais/News/Scientific_news/Articles/2014/Parkinsons-disease-a-major-breakthrough-thanks-to-gene-therapy.aspx An Anglo-French team (AP-HP, Inserm, CEA/Mircen, UPEC, Oxford Biomedica, Cambridge University) coordinated by Professor Stéphane Palfi (head of the neurosurgery department at the hôpital Henri-Mondor) conducted a gene therapy trial in 15 patients with an advanced form of Parkinson’s disease. Parkinson's disease ; Gene and cell therapy MIRcen Scientific result Fri, 10 Jan 2014 11:00:00 GMT