Frédéric Joliot Institute for Life Sciences - Sur le même thème https://joliot.cea.fr/drf/joliot/en/Pages/news/Science/2018/spectrometry-quality-control-vaccines.aspx Website of the Frédéric-Joliot Institute of Life Sciences which includes 4 departments: I2BC, DMTS, NeuroSpin and Service hospitalier Frédéric Joliot (SHFJ). The teams are mainly located at the CEA Paris-Saclay center and at the Orsay hospital. en-US https://www.cea.frhttps://www-cea-fr.admsite.extra.cea.fr/Style%20Library/Themes/drf/instituts/images/logo-cea-joliot.png Frédéric Joliot Institute for Life Sciences - Sur le même thème https://joliot.cea.fr/drf/joliot/en Improved diagnosis of acute hepatic porphyrias using mass spectrometry https://www.cea.fr/drf/joliot/en/Pages/news/Science/2024/Diagnosis-acute-hepatic-porphyrias-mass-spectrometry.aspx A collaborative study between SPI and a team from Hôpital Louis Mourier demonstrates the contribution of liquid chromatography coupled with mass spectrometry to the day-to-day analytical needs of a clinical laboratory. Application to the rapid quantification of biomarkers in acute hepatic porphyrias. Diagnosis ; Mass spectrometry ; Biomarkers SPI Scientific result ; Article Fri, 23 Feb 2024 11:00:00 GMT Functionalization of the B subunit of Shiga toxin as a vaccine tool https://www.cea.fr/drf/joliot/en/Pages/news/Science/2024/Functionalization-B-subunit-Shigatoxin-vaccine-tool.aspx Researchers at SIMoS (DMTS) describe the synthesis, engineering and evaluation of the properties of the B subunit of bacterial Shiga toxin (STxB), administered mucosally, as a vaccine tool. Their results confirm the value of using a synthetic STxB in an anti-tumor and anti-infectious vaccination strategy. Biotechnology ; Vaccine SIMOPRO Scientific result préclinique; Fri, 26 Jan 2024 11:00:00 GMT Creatine transporter deficiency: use of two original models to characterize this rare genetic disease https://www.cea.fr/drf/joliot/en/Pages/news/Science/2023/Proteins-involved-pathogenesis-creatine-transporter-deficiency.aspx Researchers at SPI (DMTS) have identified by mass spectrometry and bioinformatics analysis several biomarkers linked to the creatine transporter deficiency disorder (CTD) using two original models : mice with the clinical characteristics of CTD subjects and brain organoids derived from CTD patient cells. Mass spectrometry ; Genetic diseases ; Biomarkers ; Proteomics SPI Scientific result Thu, 22 Jun 2023 10:00:00 GMT A possible link between changes in gut microbiota and fecal SARS-CoV-2 load ? https://www.cea.fr/drf/joliot/en/Pages/news/Science/2022/Link-intestinal-microbiota-Covid-19.aspx A team from SPI used mass spectrometry to analyze the degree of alteration of the intestinal microbiota of Covid-19 patients and to look for signatures of intestinal infection by the SARS-CoV-2 virus. The level of the observed gut dysbiosis would be an indicator of the stage and extent of the infection. Mass spectrometry ; Proteomics ; Biomarkers ; Infectious diseases SPI Scientific result ; Article Thu, 12 May 2022 10:00:00 GMT ProMetIS: open bioinformatics data and tools for multi-omics phenotyping of mouse models https://www.cea.fr/drf/joliot/en/Pages/news/Science/2022/ProMetIS-open-database-multi-omics-phenotyping.aspx A consortium of researchers, led by SPI/DMTS, presents ProMetIS, a pilot study for the deep phenotyping of murine models by combining proteomic and metabolomic approaches. A significant advance for the functional characterization of genes and the development of bioinformatics integration approaches. Mass spectrometry ; Large-scale biology ; Bioinformatic SPI Scientific result Mon, 04 Apr 2022 10:00:00 GMT Biochemical analysis and mass spectrometry for the diagnosis of congenital disorders of glycosylation https://www.cea.fr/drf/joliot/en/Pages/news/Science/2022/Diagnosis-MS-congenital-disorders-glycosylation.aspx A collaboration initiated in 2017 between AP-HP (Bichat Hospital), Paris-Saclay University (Inserm 1193) and the Laboratoire d’Études du Métabolisme des Médicaments (LEMM/SPI/DMTS), leading to 4 publications in 2021, is advancing knowledge on the origin of certain congenital disorders of glycosylation, through the identification of specific biochemical biomarkers. Mass spectrometry ; Biomarkers ; Genetic diseases SPI Scientific result Tue, 04 Jan 2022 11:00:00 GMT Metaproteomics and data science for an eco-health diagnosis of soil microbiota https://www.cea.fr/drf/joliot/en/Pages/news/Science/2021/Metaproteomics-data-science-soil-microbiota.aspx Researchers from Li2D (SPI/DMTS, Marcoule) and LSCE have developed an innovative methodology for analyzing soil microbiota based on an optimal computational interpretation of metaproteomic databases, which should lead to a better understanding of the impact of pollutants on soil quality and to the prediction of their restoration potential. Environment ; Proteomics ; Mass spectrometry ; Bacteria Scientific result Thu, 07 Oct 2021 10:00:00 GMT Salmonellosis and shigellosis: a monoclonal antibody induces cross-protection https://www.cea.fr/drf/joliot/en/Pages/news/Science/2021/Salmonellosis-shigellosis-monoclonal-Ab-cross-protection.aspx Researchers at CEA-Joliot (SPI unit in collaboration with SIMoS unit) have developed a monoclonal antibody directed against a common region of two proteins of the bacteria Shigella and Salmonella and capable of inducing cross-protection against infections caused by these bacteria. Bacteria ; Vaccine Researchers SIMOPRO Scientific result Antibody;IpaD-318;host cell;injectiosome; Thu, 25 Mar 2021 11:00:00 GMT Food poisoning? Targeted mass spectrometry brings an answer https://www.cea.fr/drf/joliot/en/Pages/news/Science/2021/Food-poisoning-SE-targeted-mass-spectrometry.aspx SPI (DMTS), in collaboration with ANSES, has developed a test based on immuno-capture and targeted mass spectrometry for the quantitative and multiplex identification, in dairy products, of the eight main enterotoxins of Staphylococcus aureus, responsible for food poisoning epidemics. Mass spectrometry ; Toxicology ; CBRN-E threats Scientific result Thu, 18 Mar 2021 11:00:00 GMT Première étape vers un test quantitatif par spectrométrie de masse pour le diagnostic spécifique de la COVID-19 ? https://www.cea.fr/drf/joliot/en/Pages/news/Science/2021/Vers-test-quantitatif-MS-diagnostic-specifique-COVID-19.aspx Le LEMM, en collaboration avec le LI2D et l’AP-HP, développe un test de diagnostic spécifique de la Covid-19 par spectrométrie de masse qui permettrait aussi la quantification de la charge virale du patient. Objectif à terme : adapter la méthode pour la rendre plus sensible et compatible avec les équipements d’analyse des hôpitaux. Diagnosis ; Mass spectrometry ; Virus Researchers SPI Scientific result SARS-CoV-2; Tue, 09 Mar 2021 11:00:00 GMT SPI is committed to precision medicine for autoimmune and inflammatory diseases https://www.cea.fr/drf/joliot/en/Pages/news/Institute/2021/SPI-ATRACTion-consortium.aspx Coordinated by the Imagine Institute for Genetic Diseases, the ATRACTion program is based on a consortium of 11 academic and industrial partners, including SPI (DMTS), and aims to develop a precision medicine for patients with autoimmune and inflammatory diseases associated with primary immune deficiencies. Metabolomics ; Mass spectrometry ; Autoimmune diseases News Thu, 14 Jan 2021 11:00:00 GMT Composition of the microbiota of animals in natura: a challenge met by mass spectrometry https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Composition-microbiota-animals-in-natura-mass-spectro.aspx A team from SPI in Marcoule, in collaboration with INRAE, characterized the intestinal microbiota of a small crustacean, Gammarus fossarum, used as a sentinel of water quality in France, by combining proteogenomics and metaproteomics approaches, thus opening up interesting perspectives for the analysis of host/microbiota interactions in other animal models. Mass spectrometry ; Bacteria ; Metagenomics ; Proteomics Scientific result Mon, 24 Aug 2020 10:00:00 GMT SARS-CoV-2 detection: mass spectrometry shows its potential for rapidity. https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/SARS-CoV-2-detection-mass-spectrometry-rapidity.aspx A team from SPI in Marcoule detected by mass spectrometry, in 3 minutes and without specific reagents, signature peptides of the SARS-CoV-2 virus in clinical samples (nasopharyngeal swabs). It thus provides a proof of concept of the use of this method as a possible alternative to PCR, currently the reference method. Mass spectrometry ; Virus ; Biodetection ; Diagnosis Scientific result Thu, 06 Aug 2020 10:00:00 GMT Bacteria and archaea: a new taxonomy to better describe the unknowns? https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/New-taxonomy-bacteria-archea.aspx The discovery of myriads of new species of bacteria and archaea, linked to the explosion of metagenomics, is leading microbiologists to consider rethinking how to map certain branches of life. Tools, such as the phylopeptidomics developed at Li2D, could help them do so. Mass spectrometry ; Bacteria ; Proteomics Researchers SPI Mon, 20 Jul 2020 10:00:00 GMT Proteomic analysis of SARS-CoV-2 infected cells https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Proteomic-analysis-SARS-CoV-2-infected-cells.aspx In order to optimize the amplification of quality inactivated virus, for the manufacture of a vaccine, a team from SPI (CEA Marcoule) used mass spectrometry to analyze the dynamics of the proteome of SARS-CoV-2 infected cells, at two multiplicities of infection. With more than 3,220 identified host proteins, researchers are also starting to decipher the processes and cellular networks impacted by this virus. Virus ; Mass spectrometry ; Proteomics News Mon, 06 Jul 2020 10:00:00 GMT Mapping of Ebola T-cell response: towards a new vaccine ? https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Mapping-Ebola-T-cell-response.aspx SIMoS researchers, in collaboration with Genopole, Excellgene, Vaxeal holding SA (Switzerland), used a large-scale approach to map and characterize the response of CD4 T-cells from healthy donors, against two proteins from the Ebola-Zaire virus. They observed a strong response to the viral nucleoprotein (NP) and suggest that components of NP may be included in the design of new Ebola vaccines. Virus ; Vaccine Scientific result Wed, 24 Jun 2020 10:00:00 GMT Salmonellosis and shigellosis: first demonstration of cross-protection induced by virulence factors SipD and IpaD https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Salmonella-shigella-cross-protection.aspx SPI researchers show for the first time, in a mouse model, the induction of cross-protection against Salmonella and Shigella infections, two pathogenic bacteria responsible for gastrointestinal diseases (typhoid, dysentery among others), one of the main causes of morbidity and mortality in several regions of Africa and Asia. Vaccine ; Bacteria Scientific result Mon, 22 Jun 2020 10:00:00 GMT How the identification of attenuated forms of the SARS-CoV-2 virus could help to control the current epidemic ? https://www.cea.fr/drf/joliot/en/Pages/news/Institute/2020/Identification-attenuated-covid-virus-variants.aspx An opinion article, published in Environmental Microbiology, and written by an international consortium initiated by three researchers of Joliot Institute, proposes an original way of research on Covid-19 through the identification by sequencing of attenuated variants of the SARS-CoV-2 virus in populations at risk with little or no symptoms. Virus ; Genomics ; Vaccine Tue, 12 May 2020 10:00:00 GMT Characterization of a predictive survival biomarker in the event of septic shock https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Characterization-predictive-survival-biomarker-septic-shock.aspx Two recent studies conducted by SPI researchers, in collaboration with Inserm and AP/HP teams, reveal the value of measuring plasma levels of calgranulins as biomarkers for the prediction of mortality risk of patients with septic shock, admitted in intensive care unit. Biomarkers ; Mass spectrometry ; Infectious diseases Scientific result Tue, 24 Mar 2020 11:00:00 GMT Alzheimer's or Lewy bodies dementia? The answer with high-resolution mass spectrometry https://www.cea.fr/drf/joliot/en/Pages/news/Science/2019/Alzheimer-or-Lewy-bodies-dementia-MS-answer.aspx A team from SPI, in collaboration with the metabolic biochemistry service and the Institute of Memory and Alzheimer's Disease (IM²A) of the university hospitals Pitié-Salpêtrière, has developed a method for the simultaneous quantification of tau and α-synuclein proteins in human cerebrospinal fluid by high-resolution mass spectrometry. A promising new approach for the differential diagnosis of Lewy bodies dementia and Alzheimer's disease. Neurodegenerative diseases ; Mass spectrometry Scientific result Thu, 07 Nov 2019 11:00:00 GMT Lassa fever: a vaccine soon to be tested. https://www.cea.fr/drf/joliot/en/Pages/news/Science/2019/Lassa-fever-vaccine.aspx Researchers from Pasteur Institute, in collaboration with seven research teams, including SPI (LI2D/Marcoule), developed and evaluated the efficacy of several vaccine candidates against the virus responsible for Lassa haemorrhagic fever, endemic in Western Africa. They identified one of them as being the most effective, likely to quickly enter clinical trials in humans. Results are published in the journal Science Translational Medicine. Vaccine Scientific result Wed, 09 Oct 2019 10:00:00 GMT Mass spectrometry for quality control of vaccines https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/spectrometry-quality-control-vaccines.aspx ​A SPI Research Team (LI2D, Marcoule), in collaboration with CIRAD and AU-PANVAC, proposes a new quality control technique for veterinary vaccines, based on very high resolution mass spectrometry. This strategy, based on the use of an ad hoc database to measure the proportion of antigens and contaminants in the vaccine preparation, will improve the quality control of vaccines against contagious caprine pleuropneumonia. Mass spectrometry ; Vaccine Scientific result Thu, 05 Jul 2018 10:00:00 GMT Toxins, vaccines, biobetters: a mission at the Butantan Institute of São Paulo https://www.cea.fr/drf/joliot/en/Pages/news/Institute/2018/Toxins-vaccines-biobetters-butantan.aspx ​​Last April, a delegation of experts from the DRF visited colleagues at the Butantan Institute of São Paulo, a biomedical research center that has had close links with several researchers at the Joliot Institute since 2014. This mission is is concluded by the signing of a framework agreement between the two institutes in the field of therapeutic and vaccine innovation. Institutional ; Toxicology ; Vaccine News Fri, 25 May 2018 10:00:00 GMT Metabolomic analysis facilitated by very high resolution mass spectrometry https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/metabolomic-analysis-facilitated-by-mass-spectrometry.aspx In a study published in Analytical Chemistry, the Mass Spectrometry Research Team of the Laboratory Drug Metabolism Research Laboratory (SPI) demonstrated the interest of Orbitrap Fusion, a state-of-the-art mass spectrometer for the identification of relevant metabolites based on their masses and isotopes measured with high accuracy. Chemistry ; Metabolomics ; Mass spectrometry Scientific result Wed, 25 Apr 2018 10:00:00 GMT