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Multi-wavelength AUC - a new tool for solution studies of heterocomplexes

Vendredi 08 octobre 2021 à 11:00, Visioconférence

Publié le 8 octobre 2021
Borries Demeler
The University of Lethbridge, Alberta, Canada
In this talk I will discuss new avenues for studying complex hetero-component mixtures by analytical ultracentrifugation (AUC), involving multi-wavelength detection. With new instruments available that support multi-wavelength AUC, we can take advantage of spectral decomposition of the hydrodynamic information collected in an AUC experiment, to gain additional insights into the chemical composition of the analytes and the dynamic complexes they form in the mixture. Importantly for applications in biochemistry, we can take advantage of unique chromophores and spectral properties of different biopolymers (in particular, proteins, DNA, RNA, and lipids), as well as small organic molecules with aromatic and/or fluorescent properties. I will describe the method and illustrate the concepts by way of several systems recently studied in our lab, involving RNA loaded lipid nanoparticles (similar to the Covid-19 vaccine formulations from Pfizer/Biontech and Moderna) and other viral vector-based gene therapy formulations.

Pour suivre le webinaire : https://cnrs.zoom.us/j/95313348800

ID de réunion : 953 1334 8800
Code : 7Zq0Be

La salle des séminaires de l’IBS peut désormais accueillir 51 personnes, pour suivre le séminaire en présentiel