Our institute is composed of 5 departments
Laboratories of the Institute
Publications, Books & Publishing, Defended theses
Highlight | Structural biology
The endosomal sorting complex required for transport (ESCRT) machinery catalyzes many divergent membrane-remodeling processes such as membrane repair, enveloped virus budding and cytokinesis, among others. Researchers at IRIG have obtained the first high resolution images of the ESCRT-III complex, showing membrane-coated tubular structures that constitute a minimal machinery that can cleave membrane necks via membrane fission.
Within the protein family, ESCRT-III members are present in evolution from prokaryotes to metazoans. They polymerize on membranes in order to remodel them, often to the point of membrane fission co-catalyzed by the activity of the ATPase VPS4. Various pathologies such as cancer and neuronal deficiencies are linked to the dysfunction of the ESCRT machinery. Notably ESCRT-III and VPS4 have been associated with late steps in membrane cleavage. It is therefore important to study the structure of the ESCRT machinery within a membrane context in order to provide insight into its membrane remodeling and cleavage activity during membrane repair, budding of enveloped viruses, and cytokinesis among others.
Researchers at IRIG [collaboration with Curie Institute Paris and University of Groningen, Groningen, Netherlands] provide first high resolution cryoEM structures of ESCRT-III filaments composed of CHMP2A and CHMP3 forming membrane-coated tubular structures in vitro that resemble ESCRT-III filament architectures at virus or vesicle budding sites or the cytokinetic midbody.
Figure: Model of membrane constriction by ESCRT-III. On the left, atomic model of ESCRT-III filaments composed of a heterodimer of CHMP2A and CHMP3. The outer surface of the filaments interacts with membranes in a manner similar to the topology of membrane collars as shown in the diagram (right). VPS4 tightens the filaments that can cleave membrane necks via membrane fission.
Azad K, Guilligay D, Boscheron C, Maity S, De Franceschi N, Sulbaran G, Effantin G, Wang H, Kleman JP, Bassereau P, Schoehn G, Roos WH, Desfosses A, and Weissenhorn W
CEA is a French government-funded technological research organisation in four main areas: low-carbon energies, defense and security, information technologies and health technologies. A prominent player in the European Research Area, it is involved in setting up collaborative projects with many partners around the world.