The platform can identify and quantify fatty acids, as well as all classes of glycerolipids, from phospholipids, the main constituents of membranes in all living organisms, to galactolipids specific to photosynthetic organisms, and triacylglycerols, the major constituents of lipid droplets. It has also recently become capable of analysing sterols, including cholesterol, ergosterol and phytosterol, and pigments such as carotenoids and chlorophylls.
© Elodie Billey
Expertise and services:
- Hot lipid extraction for samples containing lipases,
- Quantification, identification and analysis of free or esterified fatty acids,
- Structural analysis of glycolipids and positioning of fatty acids on the glycerol backbone of different lipid families,
- Quantification of glycerolipids, phospholipids, galactolipids, betaine lipids and triacylglycerols,
- Quantification of sterols, cholesterol, ergosterol and phytosterols,
- Quantification of pigments, carotenoids and chlorophylls.
Resources and equipment:
- Lipid extractor,
- Fatty acid methyl ester preparation robot,
- 3 gas chromatographs coupled with flame ionisation detection and GC-FID-MS mass spectrometry,
- Ion trap mass spectrometer,
- 2 HPLC-MS/MS chains,
- HPLC chain coupled with a diode array.
How do I submit a project to the platform?
The LIPANG platform is available for internal research projects involving the Laboratory of Cellular and Plant Physiology (LPCV), collaborations funded by external parties, or industrial partnerships. For further information, please send an email to
liste-gre-lipang@cea.fr. You will receive a reply within a week. In order to understand your expectations, a telephone or video conference will be organised and, if the platform's capabilities can meet your needs, a quote will then be provided based on your requirements. LIPANG's expertise focuses on photosynthetic organisms. The platform will therefore give priority to analyses on this type of matrix, although it can work on other samples.
Project example
Analysis of the lipidome of microalgae in the Alps The ALPALGA project is one example among many supported by the Lipid team at the Laboratory of Cellular and Plant Physiology (LPCV). This team studies the response of algae to thermal stress by assessing the biodiversity of microalgae in mountainous regions and deciphering their interaction with the environment. For this project, which is also supported by the Killian Jornet Foundation, the platform established the glycerolipidome of a snow sample enriched in Sanguina Nivaloides, quantified its lipid content and determined the glycerolipidome of two new strains of Deuterostichococcus isolated in the snow. The objective is now to establish the glycerolipidome of three other strains isolated in snow and to analyse the variation in their lipid composition under different growth conditions, including light, temperature and nutrient deprivation.
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Further information : Ezzedine J.A.
et al. (2023).
Adaptive traits of cysts of the snow alga
Sanguinanivaloides unveiled by 3D subcellular imaging.
Nature Communications. 14(1):7500.
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Key words : Glycerolipid; Phospholipid; Galactolipid; Betaine lipid; Triacylglycerol; Lipidomics; Glycerolipidome; Fatty acids; Plants; Algae; Mass spectrometry; Gas chromatography; Liquid chromatography; Sterol; Pigment
Scientific Director:
Juliette Jouhet - 04 38 78 38 55 Technical Manager:Mathilde Simon TUTELLES : CEA, CNRS, INRAE, UGA
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LIPANG platform
The Cell & Plant Physiology Laboratory has an international expertise in plant lipid metabolism and in glycerolipid analysis. The lipidomics platform LIPANG makes this expertise and this skill available to company and research organizations working in the area of agronomy and agriculture, energy and health.
Developed methods meet needs in fatty acid and glycerolipid quantifications as well as lipid extraction and comprehensive lipidome mapping.
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