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Identifying exposition to low oxygen environment in human macrophages using secondary ion mass spectrometry and multivariate analysis

Publié le 29 mars 2018
Identifying exposition to low oxygen environment in human macrophages using secondary ion mass spectrometry and multivariate analysis
Auteurs
Court M., Barnes J.-P., Millet A.
Year2017-0470
Source-TitleRapid Communications in Mass Spectrometry
Affiliations
Inserm U1205, Grenoble, France, University of Grenoble-Alpes, Grenoble, France, University Grenoble Alpes, Grenoble, France, CEA, LETI, MINATEC Campus, Grenoble, France, Team ATIP/Avenir Mechanobiology, Immunity and Cancer, Grenoble, France
Abstract
Rationale: Macrophages are innate immune cells presenting a strong phenotypic plasticity and deeply involved in tissue homeostasis. Oxygen environmental tension is a physical parameter that could influence their polarizations. In this study we use time-of-flight secondary ion mass spectrometry (TOF-SIMS) to describe how various polarizations are modified by a low oxygen exposure. Methods: TOF-SIMS experiments were performed using an IONTOF ToF-SIMS 5–100 (ION-TOF GmbH, Munster, Germany). Analysis was performed using a pulsed 25 keV Bi3+ beam, sputtering was performed using a 250 eV Cs beam. Cells were fixed by paraformaldehyde before TOF-SIMS analysis. Results: Multivariate analysis of the TOF-SIMS spectra provided ion species associated with the exposure of macrophages to low oxygen concentration. We were able to obtain some species, specific of a particular polarization, advocating for the use of macrophages as reporter cells of oxygen tension in tissues. Conclusions: Our study demonstrates that macrophage molecular signature to low oxygen environment is dependent on their polarization. TOF-SIMS shows the clear capability to produce species revealing this exposition. This result opens the way to the use of TOF-SIMS as a tool to explore hypoxia in human tissues. Copyright © 2017 John Wiley & Sons, Ltd.
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ISSN9514198
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