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An oxime-based glycocluster microarray

Publié le 29 mars 2018
An oxime-based glycocluster microarray
Hoang A., Laigre E., Goyard D., Defrancq E., Vinet F., Dumy P., Renaudet O.
Source-TitleOrganic and Biomolecular Chemistry
LETI-CEA-Grenoble, 17 rue des Martyrs, Grenoble Cedex 9, France, Univ. Grenoble Alpes, CNRS, DCM UMR 5250, Grenoble, France, Ecole Nationale Supérieure de Chimie de Montpellier, 8 Rue de l'Ecole Normale, Montpellier, France, Institut Universitaire de France, 103 boulevard Saint-Michel, Paris, France
"Carbohydrate microarrays represent powerful tools to study and detect carbohydrate-binding proteins, pathogens or cells. In this paper, we report two original oxime-based methods to prepare surfaces displaying well-defined structures and valency in a given microspot with improved recognition potency with lectins. In a first ""direct"" approach, fully synthetic aminooxylated glycoclusters have been coated onto aldehyde-activated SiO2 (silicium substrate doped with 50 nm thermal oxide layer). To improve the preparation of the microarray in terms of rapidity and simplicity and to provide addressable surfaces on which sugars can be linked chemoselectively as clusters at defined plots, a second ""indirect"" strategy has been developed using successive oxime ligation steps. In both cases, binding assays with labelled lectins have revealed more potent and selective interaction due to the clustered presentation of sugars. The observed differences of interaction have been confirmed in solution by ITC. © 2017 The Royal Society of Chemistry."
Bins, Carbohydrates, Microarrays, Proteins, Sugars, Binding assays, Carbohydrate binding, Carbohydrate microarrays, Selective interaction, Thermal oxide layer, Valencies, Well-defined structures, Bioassay
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