HoloPhase 3D
3D label-free microscopy for time-lapse imaging of living organoids as they develop
HoloPhase 3D is a novel, label-free 3D microscope that works directly in a cell culture incubator. The technology is not only compact and easy to use, but also more than 10 times cheaper than standard optical microscopes.
How does it work?
A LED illumination array illuminates the sample at various angles and the resulting diffraction patterns (intensity-only measurements) are recorded using a CMOS sensor. The sample’s optical properties (refractive index and absorption) are reconstructed in 3D thanks to interconnected layers of neural networks. AI algorithms perform segmentation and classification of the reconstructed samples.
What it can do
HoloPhase 3D combines a high-performance algorithm and state-of-the-art equipment. It can be adapted to a wide range of imaging applications for in vitro diagnostics or bio-production, including:
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3D imaging for life-sciences
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Monitoring of organs-on-chips
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Imaging of human preimplantation embryos for in vitro fertilization laboratories
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Quantitative and systematic analysis of cell dynamics for in vitro 3D imaging
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Monitoring of 3D cell organization in bioreactors
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What makes it unique
The CEA-Leti team built on its strong expertise in software and integration technologies in order to develop:
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A compact, robust, low-cost and easy-to-use label-free 3D microscope that can capture images of the development of living organisms.
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What’s next?
The CEA-Leti team is currently developing:
- Correlative fluorescence and 3D refractive index imaging
- 3D real-time monitoring of cellular interaction and migration
- Coupling with microfluidics for organ-on-chip imaging
