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Published on 7 December 2023
Research FieldHealth and environment technologies, medical devices


ThemeTechnological challenges

Theme-SEngineering sciences

Health and environment technologies, medical devices Technological challenges Instrumentation Engineering sciences DRT DTBS LSIV LSIV Grenoble
Measurement of active cell nematics by lensless microscopy
At CEA-Leti we have validated a video-lens-free microscopy platform by performing thousands of hours of real-time imaging observing varied cell types and culture conditions (e.g.: primary cells, human stem cells, fibroblasts, endothelial cells, epithelial cells, 2D/3D cell culture, etc.). And we have developed different algorithms to study major cell functions, i.e. cell adhesion and spreading, cell division, cell division orientation, and cell death. The research project of the post-doc is to extend the analysis of the datasets produced by lens-free video microscopy. The post-doc will assist our partner in conducting the experimentations and will develop the necessary algorithms to reconstruct the images of the cell culture in different conditions. In particular, we will challenge the holographic reconstruction algorithms with the possibility to quantify the optical path difference (i.e. the refractive index multiplied by the thickness). Existing algorithms allow to quantify isolated cells. They will be further developed and assessed to quantify the formation of cell stacking in all three dimensions. These algorithms will have no Z-sectioning ability as e.g. confocal microscopy, only the optical path thickness will be measured. We are looking people who have completed a PhD in image processing and/or deep learning with skills in the field of microscopy applied to biology.
Département Microtechnologies pour la Biologie et la Santé (LETI) Laboratoire Systèmes d’Imagerie pour le Vivant Laboratoire Systèmes d’Imagerie pour le Vivant
ALLIER Cédric CEA DRT/DTBS/SEVI DTBS/LSIV - Bât. 42 CEA-LETI, MINATEC 17 rue des Martyrs 38054 Grenoble Cedex 9 France 0438782411
Start date1/3/2020

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