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Does the propagation of action potentials in white matter require a local increase in blood flow?


​​Using complementary imaging methods, researchers from CIEL team (BAOBAB / NeuroSpin) and the Institut de la Vision show that the propagation of action potentials in the myelinated axons of rodent optic nerves does not trigger neurovascular coupling.

Published on 23 August 2026

Neurovascular coupling

In gray matter, neurovascular and neurometabolic coupling correspond to a set of processes that allow controlling blood flow and energy metabolism according to neuronal activity.

In white matter, the energy cost of transmitting information along myelinated axons is reduced, and the need for neurovascular coupling remains unknown. Theoretical calculations suggest the hypothesis that, at least in myelinated nerves, characterized by their rarity of synapses, neurovascular coupling may not be necessary for the propagation of action potentials.

Optic nerve, a unique model of white matter

The optic nerve in rodents is a very good model of myelinated axonal tract. A team from CIEL (BOABAB/NeuroSpin)​, in collaboration with a team from the Institut de la Vision, used two complementary imaging techniques to study neurovascular coupling along the entire length of the optic nerve:

  • ​​Two-photon imaging in mice via lens implantation to visualize red blood cells and measure their veolicty in brain capillaries; ;
  • High magnetic field functional BOLD MRI (17.2 T) in rats, which allows establishing maps of transient local variations in blood flow.

The researchers observed that visual stimulations (flashing light or drifting grating) increase blood flow in the retina, the unmyelinated head of the optic nerve, and at the synaptic terminals of the nerve. However, these stimulations do not affect blood flow or oxygenation in the myelinated part of the optic nerve, i.e., the intracranial optic nerve and the optic tract.

The authors conclude that during natural visual stimulation, the propagation of action potentials in activated myelinated axons does not trigger neurovascular coupling.​

Contact at the Frédéric-Joliot Institute for Life sciences:

Luisa C​iobanu (luisa.ciobanu@cea.fr



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