To carry out their activities, Research Teams of the Frédéric Joliot Institute for Life Sciences have developed high-profile technological platforms in many areas : biomedical imaging, structural biology, metabolomics, High-Throughput screening, level 3 microbiological safety laboratory...
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Team LeadersLoïc Martinloic.firstname.lastname@example.orgHervé Nozachherve.email@example.com
To obtain sufficient amount of proteins, especially for structural analysis, our team has developed a rapid production platform of proteins at the laboratory scale in two expression formats: expression in Escherichia coli (E. coli) and in suspension human HEK293 cells. For functional analysis of proteins of interest, we have developed a variety of techniques to analyze, quantify and screen protein-protein interactions. In regards with protein engineering, different approaches could be used: one is the design of biosimilars by transferring hotspot binding domains to "miniproteins". The basis of all these technologies is the ability to generate target-directed mutagenesis coupled with the selection of the optimized mutants.
This protein expression platform in Escherichia coli (E. coli) and in suspension Human HEK293 cells includes instrumentation and expertise in the following steps:
This expression platform responds to request for protein expression of the various teams of the laboratory and works in support with the analytical mass spectrometry and crystallographic structural analysis platforms.
Different techniques are used for protein-protein interaction analysis:
Benchtop "surface plasmon resonance" and "flow cytometry" systems for protein-protein interaction analysis
CEA is a French government-funded technological research organisation in four main areas: low-carbon energies, defense and security, information technologies and health technologies. A prominent player in the European Research Area, it is involved in setting up collaborative projects with many partners around the world.