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Theses

SL-DRT-24-0567

Published on 7 December 2023
SL-DRT-24-0567
Research fieldHealth and environment technologies, medical devices

Domaine-SInstrumentation

ThemeTechnological challenges

Theme-SEngineering sciences

Field
Health and environment technologies, medical devices Technological challenges Instrumentation Engineering sciences DRT DTBS SYMS L4IV Grenoble
Title
Design of a new light-sheet microscope for the temporal monitoring of organoids-on-chip
Abstract
The subject of the thesis is the development of a fluorescence light-sheet microscope for the optical characterization of organoids-on-chips and 3D organoids. The thesis will focus on the conception of a compact multi-color 3D system, to allow time-lapse imaging of multi-scattering 3D samples directly in a cell culture incubator. The work will begin with a clear understanding of the miniaturization process on the quality of the images. The excitation will be correctly model to avoid optical artefacts and to allow the deepest penetration into the biological tissue. The candidate will be responsible to test different optical strategies as well as different excitation wavelengths. As a final step, the system will be characterized in a cell culture incubator for the morphological and functional monitoring of organs-on-a-chip and 3D organoids using specific fluorescent markers. If needed, novel modifications of the microfluidic chamber with integrated optical functions will be proposed. The research program will mainly focus on the morphological and functional monitoring of two samples: pancreatic organoids on a microfluidic chip and 3D brain organoids.
Formation
Master 2 ou Diplome d'ingénieur en optique / biophotonique Technological Research
Contact person
PAVIOLO Chiara CEA DRT/DTBS//L4IV Commissariat à l’énergie atomique et aux énergies alternatives / LETI MINATEC Campus 17 rue des martyrs F-38054 Grenoble Cedex 0438786348 chiara.paviolo@cea.fr
University/ graduate school
Université Grenoble Alpes Ecole Doctorale de Physique de Grenoble (EdPHYS)
Thesis supervisor
DRT/DTBS//PREMS
Location
Département Microtechnologies pour la Biologie et la Santé (LETI) Service des sYstèmes de Mesure pour la Santé Laboratoire Instrum optique et traitement de l’Info pour l'Imagerie In Vitro
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