To carry out their activities, Research Teams of the Frédéric Joliot Institute for Life Sciences have developed high-profile technological platforms in many areas : biomedical imaging, structural biology, metabolomics, High-Throughput screening, level 3 microbiological safety laboratory...
All the news of the Institute of life sciences Frédéric Joliot
Scientific result | Proteomics
A SB2SM Research Team (I2BC@Saclay) solved by crystallography the structure of a ternary protein complex at the interface between the nuclear envelope (emerin and lamin A / C) and the genome (BAF). In collaboration with Teams from Paris-Sud and Paris-Diderot Universities, the structure analysis, as well as in vitro interaction and proximity experiments in cellulo, suggests that a lack of interaction between the lamina A / C (nucleoskeleton) and chromatin-associated protein (BAF) may be one of the mechanisms responsible for premature aging syndromes.
Lamins are the main components of the nucleoskeleton. Whereas their 3D organization was recently described using cryoelectron tomography, no structural data highlights how they interact with their partners at the interface between the inner nuclear envelope and chromatin. A large number of mutations causing rare genetic disorders called laminopathies were identified in the C-terminal globular Igfold domain of lamins A and C. We here present a first structural description of the interaction between the lamin A/C immunoglobulin-like domain and emerin, a nuclear envelope protein. We reveal that this lamin A/C domain both directly binds self-assembled emerin and interacts with monomeric emerin LEM domain through the dimeric chromatin-associated Barrier-to-Autointegration Factor (BAF) protein. Mutations causing autosomal recessive progeroid syndromes specifically impair proper binding of lamin A/C domain to BAF, thus destabilizing the link between lamin A/C and BAF in cells. Recent data revealed that, during nuclear assembly, BAF's ability to bridge distant DNA sites is essential for guiding membranes to form a single nucleus around the mitotic chromosome ensemble. Our results suggest that BAF interaction with lamin A/C also plays an essential role, and that mutations associated with progeroid syndromes leads to a dysregulation of BAF-mediated chromatin organization and gene expression.Read the French version
C Samson, A Petitalot, F Celli, I Herrada, V Ropars, MH Le Du, Naïma Nhiri, Eric Jacquet, Ana-Andrea Arteni, Brigitte Buendia, S Zinn-Justin. Structural analysis of the ternary complex between lamin A/C, BAF and emerin identifies an interface disrupted in autosomal recessive progeroid diseases. (2018) Nucleic Acids Res. http://dx.doi.org/10.1093/nar/gky736
CEA is a French government-funded technological research organisation in four main areas: low-carbon energies, defense and security, information technologies and health technologies. A prominent player in the European Research Area, it is involved in setting up collaborative projects with many partners around the world.