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Scientific result | Mass spectrometry | Vaccine
A SPI Research Team (LI2D, Marcoule), in collaboration with CIRAD* and AU-PANVAC**, proposes a new quality control technique for veterinary vaccines, based on very high resolution mass spectrometry. This strategy, based on the use of an
ad hoc database to measure the proportion of antigens and contaminants in the vaccine preparation, will improve the quality control of vaccines against contagious caprine pleuropneumonia.
Vaccines to protect livestock against contagious caprine pleuropneumonia (CCPP) consist in inactivated, adjuvanted antigens. Quality control of these vaccines is challenging as total protein quantification provides no indication of protein identity or purity, and culture is not an option. Here, a tandem mass spectrometry approach was used to identify the mycoplasma antigen contained in reference samples and in commercial CCPP vaccines. By the same approach, the relative amounts of mycoplasma antigen and residual proteins originating from the production medium were determined. Mass spectrometry allowed easy and rapid identification of the peptides present in the vaccine samples. Alongside the most probable mycoplasma species effectively present in the vaccines, a very high proportion of peptides from medium constituents was detected in the commercial vaccines tested.Read the French version.*CIRAD : French Agricultural Research Centre for International Development**AU-PANVAC : Pan African Veterinary Vaccine Center, African Union Commission, Ethiopia
Thiaucourt F, Pible O, Miotello G, Nwankpa N, Armengaud J. Improving quality control of Contagious Caprine Pleuropneumonia vaccine with tandem mass spectrometry. Proteomics, 2018, http://dx.doi.org/10.1002/pmic.201800088
CEA is a French government-funded technological research organisation in four main areas: low-carbon energies, defense and security, information technologies and health technologies. A prominent player in the European Research Area, it is involved in setting up collaborative projects with many partners around the world.