Activities
- Determination of biomarkers in brain samples or cell culture extracts, models of neurodegenerative diseases (electrophoresis and western blotting, ELISA, determination of enzyme activities).
- Primary cell cultures (of neurons, astrocytes or mixed cultures) and cell lines. Videomicroscopy analyses, toxicity tests (MTT, LDH, fluorescein).
- Flow cytometry
- Development and production of viral vectors (lentiviruses and AAV).
Evaluation of the biodistribution and biological effects of these vectors are then carried out principally by the histology platform, but also by the
imaging platform (PET imaging) and the
behavioral analysis platform.
Equipments
- Well equipped biosafety level 2 and 3 culture rooms (incubators, biosafety cabinets, binocular microscopes, inverted fluorescence microscopes including a videomicroscopy system)..
- Chemiluminescence detection system for western blots (Fusion FX7).
- Multiwell plate reader for the measurement of fluorescence, luminescence and absorbance.
- Three-laser cytometry.
Biosafety level 3 laboratory.
(© Stroppa/CEA)