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Highlight | Scientific publications | Scientific result | Brain | Health ＆ life sciences | Positron Emission Tomography
The development of ligands for positron emission tomography (PET) studies of neuroinflammation is attracting immense scientific and clinical interest. Researchers of MIRCen laboratory combined their expertise to present a proof of concept on how multimodal 3-D analysis can evaluate the diagnostic performances of different PET quantification methods of [18F]DPA714, a widely used PET imaging ligand for neuroinflammation.
plays a central role in a variety of neurological diseases. Over the last
decade, PET imaging of neuroinflammation proved useful for non-invasive
identification and quantification of the inflammation cascade. [18F]DPA714 is the
most promising ligand pushed forward into clinical studies. This radiotracer
binds with a high specificity to the 18kDa Translocator Protein (18kDa TSPO), a
molecular biomarker of neuroinflammation. Nevertheless, quantification of
TSPO-PET remains a topic under debate because of the complex binding properties
of TSPO-PET ligands. For clinical practice, fully non-invasive quantification
methods without any additional blood measurements are preferable, but their
diagnostic performance has never been compared.
of the CNRS Laboratory of Neurodegenerative Diseases at MIRCen created an
animal model with controlled and localized TSPO expression using a viral-vector
strategy, on which they performed a [18F]DPA714 PET scan before 3-D histological
staining of TSPO in the entire brain. The PET images were analyzed according to
9 different quantification methods. When
compared to the reference TSPO-histological 3D brain, each PET voxel was
classified as true or false, positive or negative. Using this approach, it was
shown that standard uptake value ratios using the cerebellum as a reference
region has the most optimal score amongst all non-invasive quantification
study will help guide future clinical studies that increasingly use PET-TSPO
Immunohistochemical analysis of the lenti-CNTF
NHP model. Immunostainings for TSPO (a, b) and VIM (d, e) were rendered
three-dimensional, which allowed to appreciate a major overlap between VIM-IHC
(f) and TSPO-IHC (c). Higher magnifications confirmed that TSPO-IHC was only
detected in activated astrocytes (g, h, i), and not in the microglia (j, k, l).
Assessment of simplified methods for quantification of [18F]-DPA-714 using 3D whole-brain TSPO immunohistochemistry in a non-human primate | Journal of Cerebral Blood Flow & Metabolism
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