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Cytometry and Cell Sorting

Published on 19 December 2022
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The Cytometry​ and Cell Sorting facility at the Institute of Cellular and Molecular Radiation Biology (CEA) provides instrumentation and technical expertise, for the development of collaborative projects involving flow cytometric analysis and cell sorting.


Cytométrie et tri Cellulaire



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  Jan BAIJER
  Principal investigator​
  Phone : 33 (0)1 46 54 97 51


Secretary 

Aurélie GOURET
Phone : +33 (0)1 46 54 98 66


​Know-how

  • Analysis and cell sorting​
      • ​​Examination, counting and sorting of fluorescencetagged cells or particles.
      • ​​Multi-laser configuration for multicolor cytometry applications (5 lasers/16 colors).​

      • PF cyto 3.pngCell sorting
        • ​Separation of cells or particles groups of interest by electrostatic deflection. Collection of up to 4 fractions in a single experiment.​
        • ​High speed cell sorting (50,000 cells per second) for the selection of rare populations (< 0.01%; e.g. stem cells).
    PF cyto 4.png






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    ​Applications and projects


      • Research and DevelopmentPAR-20110718-013.img.jpg
        • ​ Biological sample analysis (e.g. blood, cell culture)
        • ​Separation of cell populations for further analysis and use​
      • Current programs
        • Stem cells and regenerative medicine: hematopoietic, neuronal and gonadal stem cells analysis and sorting for research and development. Applications in regenerative medicine (stem cell grafting to reconstruct damaged tissues or organs).

        • ​Radiosensitivity: current research foc​uses on intrinsic and hereditary radiosensitivity, assessing apoptosis rates in lymphocyte subpopulations on samples from related individuals belonging to large kindred-families

    ​Equipments and charateristics

    • PF cyto 1.png
      • 5 cytometers for analysis (LSR II SORP, Canto, CyAn, et FACSCalibur) 






      • PF cyto 2.png3 cytometers for analysis and cell sorting (Influx, FACS Aria SORP and 
MoFlo) 
      • Safety facilities (L2) adapted to biohazard applications.
      • Dedicated and high experienced staff.
      • Results confidentiality




    Authorship and Results ownership​​​
    Significant contribution to the project should be acknowledged in the publication.

    Results can lead to patent filing. Ownership shall be discussed regarding the involvement of each partner and terms of the contract set up.

    Publications