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Cytometry and Cell Sorting

Published on 13 October 2023
The Flow Cytometry platform at the Institut de Radiobiologie Cellulaire et Moléculaire (CEA) offers its instrumentation and expertise for the development of collaborative projects based on flow cytometry and cell sorting.

Cytométrie et tri Cellulaire 

Platform Co-Manager
Phone: 33 (0)1 46 54 71 50
 Platform Co-Manager
  Phone : 33 (0)1 46 54 97 51


Aurélie GOURET
Phone : +33 (0)1 46 54 98 66


  • Cell Analysis​
      • Analysis of heterogeneous samples of fluorescently-labeled particles.
      • Multi-laser configurations for multi-color applications (5 lasers/up to 40 colors).
      • Autonomous use by researchers approved by the platform.

    • PF cyto 3.pngCell sorting

      • Separation of cells or particles of interest by electrostatic deflection. Collection of up to 4 fractions per sample.
      • Aria Sorp FACS sorter. Autonomous sorting by teams.
      • High-speed Influx sorter (up to 50,000 events per second) for selection of rare subpopulations (< 0.01%; e.g. stem cells). Sorting carried out by the Platform in collaboration with the teams.

      ​Applications and projects

        • Research and DevelopmentPAR-20110718-013.img.jpg
          • ​ Analysis of biological samples (e.g. blood, bone marrow, cell culture).
          • Sorting of cell populations for analysis (Single Cell, RNA or DNA, transplantation, culture).
        • Current programs
          • Analysis and sorting of hematopoietic, neural and gonadal stem cells for various research programs. Applications in radiobiology, oncology, regenerative medicine (stem cell transplantation for tissue or organ reconstitution).

          • ​Intrinsic and hereditary radiosensitivity, through analysis of radiation-induced apoptosis and transcriptome in lymphocyte subpopulations of related individuals from large families.

      ​Equipments and Specifications

      2 conventional cytometers (LSR II SORP, Canto).

      PF cyto 1.png

      1 spectral cytometer
      (Aurora Cytek)

      2 cytometers for cell analysis and sorting
      (Influx, FACS Aria SORP).

      PF cyto 2.png

      • The Platform laboratory complies with containment standards (L2).
      • ​Sorters are installed under a filtering hood adapted to biohazard applications.
      • Each cytometer is assigned an experienced referent:

        • Confidentiality of results.​
          • All experimental data is permanently stored on CEA internal servers with restricted access.

      How to find us
      Use of the equipment is open to all research teams who request it.
      Such use will be invoiced.
      All requests for use, rates and access to the site must be addressed to the  Cytometry Platform