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CyACT

Cytometry, Cell Analysis and Sorting

Plateforme de Cytométrie et Tri Cellulaire

Published on 22 January 2026
​​
The Cytometry, Cell Analysis and Sorting platform at the Department of Cellular and Molecular Radiobiology​ (DRCM, CEA) offers its instrumentation and expertise for the development of collaborative projects based on flow cytometry and cell sorting.​​


​​​​​​
Tharshana STEPHEN
Platform Manager
Phone: 33 (0)1 46 54 83 58
  
Nathalie DECHAMPS
Technician
Phone : 33 (0)1 46 54 72 39

​Secretary
Aurélie GOURET
Phone : +33 (0)1 46 54 98 66
secretariat-u1274@cea.fr


​Offers and services
  • Cell Analysis​
    • Analysis of heterogeneous samples of fluorescently-labeled particles.
    • Multi-laser configurations for multi-color applications (5 lasers/up to 40 colors).
    • Autonomous use by researchers approved by the platform.







  • Cell sorting
    • Separation of cells or particles of interest by electrostatic deflection. Collection of up to 4 fractions per sample.
    • Aria Sorp FACS sorter. Autonomous sorting by teams.
    • High-speed Influx sorter (up to 50,000 events per second) for selection of rare subpopulations (< 0.01%; e.g. stem cells). Sorting carried out by the Platform in collaboration with the teams.

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​Applications and projects

  • Research and Development
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    • ​ Analysis of biological samples (e.g. blood, bone marrow, cell culture).
    • Sorting of cell populations for analysis (Single Cell, RNA or DNA, transplantation, culture).

  • Current programs
      • Analysis and sorting of hematopoietic, neural and gonadal stem cells for various research programs. Applications in radiobiology, oncology, regenerative medicine (stem cell transplantation for tissue or organ reconstitution).

      • ​Intrinsic and hereditary radiosensitivity, through analysis of radiation-induced apoptosis and transcriptome in lymphocyte subpopulations of related individuals from large families.
​​

Equipments and resources

2 conventional cytometers (LSR II SORP, Canto).

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1 spectral cytometer
(Aurora Cytek)


2 cytometers for cell analysis and sorting
(Influx, FACS Aria SORP).

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  • The Platform laboratory complies with containment standards (L2).
  • ​Sorters are installed under a filtering hood adapted to biohazard applications.
  • Each cytometer is assigned an experienced referent:

Confidentiality of results​​​​​

  • All experimental data is permanently stored on CEA internal servers with restricted access.

How to find us
Use of the equipment is open to all research teams who request it.​​
Such use will be invoiced.
All requests for use, rates and access to the site must be addressed to the  CyACT@cea.fr​