The technological laboratory "Immunology and Infectiology" (L2i) of IDMIT Department focuses its activities on the analysis of the immune response to infection and vaccination, especially on:
- the dynamics of pathogen dissemination in the host
- the immune response to human pathogens and vaccines
- the cross-talk of these parameters at the molecular and cellular level to contribute to the development of new vaccine and therapies for human infections and immune disorders.
L2I activities © IDMIT / CEA
We offer technological core facilities dedicated to:
Quantification of human pathogens up to BSL-3 level by quantitative real-time PCR (qPCR) and classical bacteriology
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Multiplex analysis of biomarkers like cytokines using Bio-Plex 200 technology (up to 38 parameters in routine assays)
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Multiplex analysis of antibody responses using electrochemiluminescent assays (MesoScale Discovery SQ120 MM), classical ELISA and functional assays : neutralization, antibody-dependent cell-mediated cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP) and complement-dependent cytotoxicity (CDC)
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ELISPOT and FluoroSPOT T-cell and B-cell assays
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Integrated data analysis
|  Pre-clinical development of a vaccine candidate against HIV A. T-cell response immune response to the vaccine candidate analyzed by IFN-γ ELISPOT B. virus distribution in tissues analyzed by qPCR C. comparison of viral load in tissues of vaccinated versus control animals (* p<0.05, ** p<0.001, Mann-Whitney test)
SFC : spot-forming cells, PBMC : peripheral blood mononuclear cells, p.i. : post immunization ; AUC : area under the curve ; SHIV : chimeric HIV/Simian Immunodeficiency Virus.
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